Rapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity

Authors

  • A Razei Bqiyatallah University of Medical Sciences
  • H Aghamollaei Bqiyatallah University of Medical Sciences
  • SL Mousavi Shahed University
Abstract:

Introduction: Campylobacter jejuni is one of the most common causes of food poising in humans. Rapid and specific detection of these bacteria has an important role in diagnosis and treatment of infection. The aim of this study was to design a specific PCR for the detection of Campylobacter jejuni. Methods: In this experimental study, oxidoreductase gene from the Campylobacter jejuni was selected for rapid and specific detection. For this purpose, specific primers were designed and charecterized by bioinformatics software. Bacterial genome was extracted by phenol-chloroform method and PCR was optimized to obtain a specific product. Specificity of the designed reaction was investigated using six bacterial species. The sensitivity of the PCR reaction was calculated by the serial dilutions method. Results: The designed primer was specific to oxidoreductase gene of Campylobacter jejuni and after optimization, a unique 167-bp band was amplified. This primer was specific to Campylobacter jejuni and did not show any cross reaction with other bacterial genomes. The detection limit of this reaction was 5 pg of genomic DNA. Conclusions: The optimized PCR used in this study was a rapid, sensitive, and specific method for detection of Campylobacter jejuni. For confirmation of this method, detection of C. jejuni from food samples is proposed.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Assess the prevalence rate of Campylobacter genus and Campylobacter jejuni species in raw milk collected from the Amol City by Multiplex- Polymerase Chain Reaction

Background & Objective: Campylobacter can be transmitted through the raw milk. The purpose of this study was to determine the prevalence of Campylobacter genus and Campylobacter jejuni (C. jejuni) species in raw milk samples. Materials & Methods: In this study, 72 samples of raw milk were collected of the platforms milk in the Amol city in summer. Phenotypic identification of Campylobacter genu...

full text

RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SPECIMENS BY POLYMERASE CHAIN REACTION

We investigated the use of DNA amplification by polymerase chain reaction (peR) for detection of Mycobacterium tuberculosis in 300 patients who were suspected of having pulmonary tuberculosis and compared the results with culture results which were performed in parallel with PCR. Two-thirds of each sample was processed for smear and culture by standard methods and one-third was prepared fo...

full text

Direct Polymerase Chain Reaction Detection of Campylobacter jejuni and Campylobacter coli in Raw Milk and Dairy Products.

[This corrects the article on p. 2161 in vol. 59.].

full text

The Development of Real-time Polymerase Chain Reaction for the Detection of Campylobacter Jejuni

full text

Polymerase chain reaction method for the rapid detection of virulent Shigella spp.

Bacillary dysentery, or shigellosis, is a disease of humans in which the colonic epithelium is invaded by bacteria and subjected to inflammatory destruction. The aim of this study was to develop a polymerase chain reaction(PCR) test for detection of virulent Shigella spp.. For this purpose, the primers were designed to amplify a 526-bp internal region of the Shigella spp. icsA gene, which encod...

full text

the study of practical and theoretical foundation of credit risk and its coverage

پس از بررسی هر کدام از فاکتورهای نوع صنعت, نوع ضمانت نامه, نرخ بهره , نرخ تورم, ریسک اعتباری کشورها, کارمزد, ریکاوری, gdp, پوشش و وثیقه بر ریسک اعتباری صندوق ضمانت صادرات ایران مشخص گردید که همه فاکتورها به استثنای ریسک اعتباری کشورها و کارمزد بقیه فاکتورها رابطه معناداری با ریسک اعتباری دارند در ضمن نرخ بهره , نرخ تورم, ریکاوری, و نوع صنعت و ریسک کشورها اثر عکس روی ریسک اعتباری داردو پوشش, وثی...

15 صفحه اول

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 24  issue 1

pages  56- 62

publication date 2017-06

{@ msg @}

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023